FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences46494701
Sequences flagged as poor quality0
Sequence length76
%GC39

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1538340.3308635106611396No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG486970.10473666665799185No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT751150.061.9067421
GTGAGGT642750.048.95441
GATCGTA411050.044.9075552
GATCGTC187350.043.5672532
ACCGCAT273900.043.4454881
GATCGTT276050.042.8260772
TGAGGTA352450.041.180012
CCGCATA137500.039.3554232
GATCGTG236850.037.240722
CCGCATT85500.032.7043652
TGAGGTC202650.030.8270572
ATCGTAG131250.025.4431783
CATTCAG391800.024.86082333
GAGGTAA240300.024.4318223
ATCGTAT236900.024.2680033
CACATTC413250.024.19549431
GTCACAT416500.024.07686229
ATCGTAA312900.023.7598883
GAGGTAT212150.023.734353
CCGCATG93550.023.338882
Produced by FastQC (version 0.11.5)