FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences677278
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCACC3407.3035335E-712.35292870
CAGTCAC3551.2305354E-611.83097469
TCACCGC2550.0013556210.98038170
GTCACCG2550.0013556210.98038169
CCAGTCA4351.399318E-59.65516368
ACTCCAG6857.2486728E-99.1970770
TCCAGTC5251.2727619E-47.99999167
ACACGTC17600.07.755674470
CTCCAGT6452.1384201E-57.59689170
GTCTGAA13001.2732926E-117.26922370
CACACGT19150.07.127929769
GAACTCC8906.0341335E-77.078644370
CGTCTGA12908.367351E-117.05425669
TCTGAAC11008.511051E-96.99999370
ACGTCTG13906.730261E-116.798553570
GCACACG20100.06.616908668
ATGTCAT8501.6550403E-56.588228713
AACTCCA9401.0637385E-56.329780669
CACGTCT16053.3105607E-106.105912769
CTGAACT10509.0744525E-65.999993368
Produced by FastQC (version 0.11.5)