FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences904177
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAT2200.005667466711.13635469
TCACCGC3853.889609E-59.99999269
GTCACCG3853.889609E-59.99999268
AGTCACC5251.3436766E-69.33332670
CGTCTGA16950.08.67255969
GTCTGAA17600.08.55112970
CAGTCAC5805.173606E-68.44826969
TCCAGTC7301.7197417E-67.671226567
ACGTCTG19200.07.65624468
CCAGTCA6108.836495E-57.459010668
CACGTCT20750.07.253006567
CTGAACT14001.0913936E-116.999994370
AGCACAC29750.06.82352470
TCTGAAC14907.2759576E-126.812075669
GAACTCC10905.2928954E-86.74311470
ACACGTC22600.06.659286566
AGAGCAC36200.06.187840570
GAGCACA32950.06.16084569
CTCCAGT8255.329783E-45.939389769
CACACGT24850.05.915488265
Produced by FastQC (version 0.11.5)