FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP63_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP63_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences753648
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCCGG200.00695113852.4999541
CCAGTCA5701.8189894E-1212.2806970
TCCAGTC6005.456968E-1211.66665669
CAGTCAC4652.1049345E-811.29031270
CTCCAGT6907.4578566E-1110.14491868
ACTCCAG7503.6379788E-119.79999167
TGAACTC11450.08.25326770
TCTGAAC12050.08.13277268
CTGAACT11203.6379788E-128.12499269
GAACTCC9302.4811015E-97.90321869
GCACACG20500.06.99999470
AACTCCA10351.471999E-76.763278566
CGTCTGA13753.7471182E-106.618175566
CAAACTC5300.0098774966.603767430
AGCACAC23250.06.47311270
GTCTGAA14106.712071E-106.45389467
CACGTCT16055.0931703E-116.32398264
ACGTCTG14501.2805685E-96.275855565
ACACGTC18153.092282E-115.97795670
GAGCACA25350.05.936877769
Produced by FastQC (version 0.11.5)