FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences974026
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCCGCC1250.001762332916.80000150
ACCGCTC3151.8515493E-814.44444570
CGTTATC1500.005934375314.00000144
TCACCGC3702.0286534E-611.35135268
CACCGCT3702.0286534E-611.35135269
GTCACCG4005.164313E-610.567
TCCAGTC7858.913048E-119.36305770
CTCCAGT8901.2732926E-119.04494470
ACTCCAG10102.1827873E-118.31683269
TAATAGG3900.00485114758.0769232
AGTCACC4955.9530797E-47.77777866
TCTTGCA5006.628938E-47.700000321
GAACTCC12252.5465852E-117.428570767
CCAGTCA6702.8052035E-46.79104564
CAGTCAC6300.00103472216.66666770
CTGAACT15256.311893E-106.19672170
CGTCTGA17154.3655746E-116.12244970
TCTGAAC16601.200533E-106.11445870
TGAACTC15053.0195224E-96.04651166
AACTCCA14609.813448E-95.993150768
Produced by FastQC (version 0.11.5)