FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences939127
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCTGC1150.001007841118.26084332
CACCGCT2750.00254319810.18180270
TCCAGTC6907.4578566E-1110.14491270
CAGTCAC5201.08831955E-710.09613870
TCACCGC3100.00683731739.03224469
CCAGTCA5856.0223283E-78.97434569
AGTCACC4108.009714E-48.53657270
CTCCAGT7604.2753527E-88.2894669
TGAACTC14000.08.24998870
CTGAACT14000.08.24998869
TCTGAAC15000.07.93332168
GTCTGAA16700.07.54489967
ACTCCAG8553.102159E-77.3684168
CACGTCT20050.07.15709670
CGTCTGA16200.07.129618666
AGAGCAC33100.06.873100870
GAACTCC11301.4622856E-86.81414969
ACGTCTG17350.06.6570565
ACACGTC21500.06.511617770
GCACACG24900.06.184729670