FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP10_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP10_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences735998
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGGCAC850.00378978720.58795511
GCCCCAT950.007208763618.42080311
CACGGTT1000.00968791517.49976257
AGTCACT4451.6505983E-610.22527568
TCCAGTC6101.0748408E-89.75462870
TCACTCC4005.9085447E-59.62552470
GTCACTC4107.74105E-59.39075569
CTCCAGT6755.631591E-99.33384169
CAGTCAC4653.076186E-59.03274867
CCAGTCA5401.927021E-58.42638470
ACTCCAG7755.1313145E-77.677836470
CGTCTGA14150.07.42089870
CACACGT20000.07.350470
GAACTCC9805.4200427E-87.14324570
ACGTCTG15400.07.04583869
GCACACG21000.07.000380569
ACACGTC18450.06.639927470
CACGTCT16653.6379788E-126.516870568
TCTGAAC13802.8067006E-96.340924369
CTGAACT12852.7888746E-86.264931770
Produced by FastQC (version 0.11.5)