FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_SBP_HALO_bZIP10_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_SBP_HALO_bZIP10_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences930021
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCA6703.6379788E-1210.9700970
CCCAATC3055.1671267E-410.32781334
CACTCCG2800.0029530879.99994770
TCCAGTC7706.002665E-119.54540369
CTCCAGT9607.2759576E-128.74995370
ACTCCAG10656.730261E-117.887281470
GTCACTC5000.00562701376.999962369
TCACTCC5000.00562701376.999962370
AGTCACT5750.00291558056.695616268
CAGTCAC5850.00349339876.581161567
ACGTCTG19350.06.51159370
GAACTCC13551.877197E-96.457529568
CACGTCT21550.06.17166169
CGTCTGA18255.456968E-126.136953470
CACACGT25650.06.003866770
CTGAACT15801.4624675E-95.98098167
AACTCCA15901.6971171E-95.94336469
GCACACG26450.05.82227569
TGAACTC15758.507413E-95.777746767
AGCACAC30300.05.775546670
Produced by FastQC (version 0.11.5)