FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_pIX_HALO_Nis_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_pIX_HALO_Nis_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2172127
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG1851.4551915E-1124.59457853
GTCACAT13650.023.33331729
CATTCAG14450.021.31486733
GCGGGGT1859.980795E-920.81079752
CAGTCAC15600.020.19229527
TGGCGGG2453.092282E-1119.99998950
AGTCACA15650.019.680528
CACATTC17200.018.92440631
CCAGTCA17950.017.74372126
TCCAGTC18850.016.71086525
CTCCAGT18900.016.4814724
ATGGGGG6000.015.16665642
TGCCGGG2553.9056795E-715.0980351
TCACATT22600.014.86724830
ACTCCAG21100.014.59714723
ATTCAGA22700.014.18501234
ACATTCA23700.014.17720632
ATCGGGG2505.268521E-613.99999142
TTCAGAA23850.013.7945435
TCAGAAA24800.013.2661236
Produced by FastQC (version 0.11.5)