FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_SBP_bZIP53_pIX_HALO_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_SBP_bZIP53_pIX_HALO_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences731250
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGTG500.00720871627.99997347
TGGTCGC850.003789486420.58821570
TTGCGAC1000.00968715617.4999832
TCCGGAG1901.13302674E-414.73682969
CAGTCAC4202.1827873E-1114.16665470
GTACTAC1500.00593254713.99998732
GGGGAGA1850.001547294913.2432312
CCGGAGA2601.1752544E-412.11537370
CCAGTCA5004.0381565E-1011.89998869
TCCAGTC5401.4479156E-911.01850768
CCATTGG2300.00788413910.652163555
CTCCAGT6603.8831786E-89.01514267
CTGGTTT3500.00182107218.99999128
ACTCCAG7705.324E-88.1818170
GAATTGG4150.0084625467.59035440
ACGTCTG14101.8189894E-127.198574569
CGTCTGA13252.1827873E-117.13206870
GTCTGAA12904.4219632E-96.511621570
GAACTCC9702.49828E-66.494838768
CACGTCT15158.0035534E-116.46864168
Produced by FastQC (version 0.11.5)