FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP9_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP9_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4410138
Sequences flagged as poor quality0
Sequence length76
%GC38

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG5100.015.78430144
TCGGGGG5050.015.24751443
ATCGGGG4750.014.7368342
TAGGGGC4005.177777E-610.4999941
GTCCGGG3351.0567199E-410.44775359
CGTCCGG3854.2861624E-49.09090258
AATCGGG9252.910383E-118.70269541
ACGTCCG4602.7116115E-48.36955857
CACTCCG18000.08.36110469
TCCGGGT4653.0471047E-48.27956360
ACTCCGG17800.08.06179170
AACGTCC7402.1197047E-67.567561656
TCCGGAG17400.07.241373570
AGGGGCA4950.00511260847.070701642
CAGTCAC34200.06.95905970
CTCCGGA19650.06.946559469
CCCGATA6107.186552E-46.885240621
GCGGGTC6500.00147727116.46153352
GAGCACC7601.8553514E-46.447363470
TTAGGGG7105.699279E-46.40844640
Produced by FastQC (version 0.11.5)