FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4377810
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG4651.6461854E-912.04300842
TCGGGGG5351.6547165E-79.81308143
CGGGGGG6151.2757482E-79.10568844
TCCGGAG18150.08.29200870
ACTCCGG18350.08.201632570
CTCCGGA18350.08.01089769
CACTCCG21250.07.905879570
CAGTCAC36800.07.60869369
CCAGTCA42450.07.58539270
GAGCACC9857.6379365E-97.46192770
TCCAGTC48350.07.02171469
GTCACTC31750.06.83464470
CTCCAGT54000.06.805553470
AGTCACT39900.06.754383670
ACTCCAG57450.06.579632369
TCACTCC29750.06.35293970
GAACTCC72350.06.33724770
TGAACTC88450.06.21254770
AATCGGG8601.3812576E-46.10464941
CTGAACT92200.06.0357969
Produced by FastQC (version 0.11.5)