FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996585
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGGGG2952.895365E-511.86438942
ACTCCGG10500.09.66665270
TGGGGGG4653.0857664E-59.0330361
CACTCCG10950.08.94975969
CGGGGGG4653.0469403E-48.27955844
GTCACTC20000.07.874988670
ACTCCAG37550.07.73634170
TCCAGTC30100.07.67440770
TCGGGGG4200.00943576457.499989543
CAGTCAC23650.06.955592668
CTCCGGA13201.4733814E-106.893929570
CTCCAGT35000.06.49999169
GAGCACC7004.797514E-46.49999170
GAACTCC46250.06.356747668
AGTCACT27100.06.328404469
CCAGTCA26650.06.30393167
TGAACTC57650.06.19253270
CTGAACT56450.06.076164769
ACGTCTG71050.06.009843370
TCCGGAG12952.1620872E-75.945937670
Produced by FastQC (version 0.11.5)