FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP10_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_SBP_bZIP53_HALO_bZIP10_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6529313
Sequences flagged as poor quality0
Sequence length76
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGGG9700.020.2060844
TCGGGGG9500.019.52621343
ATCGGGG9450.018.8887942
GACCGCC7750.013.09670533
AATCGGG15550.011.92919741
CCGCCCA8350.011.317306535
TGACCGC8850.010.6779132
TAGCGTC9200.010.65225
AGGGGGG7909.094947E-129.7478291
AATAGCG12100.09.5454783
ACCGCCC9701.8189894E-129.02057134
GTAGGCG8403.4197E-108.74995448
ACTCCGG39100.08.50379170
GAATCGG23250.08.43006340
ATAGCGT11950.08.2008574
CGTCAGG13450.08.0668728
CACTCCG41250.08.06056469
AGCGTCA14600.07.9109786
GCGTCAG14250.07.8596077
TCCAGTC91750.07.476870
Produced by FastQC (version 0.11.5)