FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_PpPLATZ_Nis_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_PpPLATZ_Nis_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9886466
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG22050.038.09519253
GCGGGGT20650.037.96605752
CATTCAG161000.035.21734633
AATCGCG17250.034.6956141
TGCGGGG18100.032.87288751
CACATTC176150.032.12882631
TTGCGGG20550.032.01942450
GTCACAT177750.031.83962429
CAGTCAC186150.030.47807327
AGTCACA187450.030.30404728
TTCAGAA191900.029.67427435
ATTCAGA194500.029.25960534
ATGGGGG44150.028.38048642
ACATTCA203700.028.23020732
ATCGGGG20250.028.17280642
TCAGAAA197650.027.90788336
ATCGCGT10350.027.72943542
CCAGTCA205500.027.55714226
TGGCGGG25200.027.36107850
CAGAAAT201600.026.9270537
Produced by FastQC (version 0.11.5)