FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_PpPLATZ_Nis_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_PpPLATZ_Nis_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10611747
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG23400.040.5341653
GCGGGGT23750.039.7894552
CATTCAG191100.036.7948533
AATCGCG20250.036.12343241
TGCGGGG21200.035.8254551
ATGGGGG55100.033.1578742
CACATTC213400.032.98264331
TTGCGGG23800.032.94115450
ATCGCGG14850.032.7609242
GTCACAT215850.032.51098629
ATCGGGG20600.031.60192342
TGGCGGG26300.031.53990450
TTCAGAA226150.031.23146435
AGTCACA225650.031.19209328
AATGGGG67200.030.88539741
CAGTCAC227250.030.81846227
ATTCAGA231000.030.56058934
TCAGAAA226700.030.44550336
CAGAAAT229850.029.32780537
ACATTCA242750.029.25435832
Produced by FastQC (version 0.11.5)