FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_PpPLATZRI_Nis_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_PpPLATZRI_Nis_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7418494
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATTCAG93750.032.44259333
AATCGCG11750.031.57439841
GCGGGGT10900.029.2201252
CACATTC106200.028.57338331
CGGGGTG12250.028.57136353
GTCACAT105850.028.535629
CAGTCAC108100.027.97403527
AGTCACA110500.027.39812928
TTGCGGG11950.026.94554750
TGCGGGG10350.025.70042651
ATTCAGA120950.025.23351134
TTCAGAA121950.025.17009735
CCAGTCA120850.024.96477726
ATCGGGG13250.024.83013542
ACATTCA125300.024.38542232
ATCGCGT7050.024.32618542
TCCAGTC124750.024.01597825
TCAGAAA127750.023.56159236
GAAATCG40650.023.33328239
ATCGCGG8450.023.19521742
Produced by FastQC (version 0.11.5)