FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_PpPLATZRI_Nis_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_PpPLATZRI_Nis_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8979432
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGTG16700.033.95202653
GCGGGGT15850.032.23968552
CATTCAG135700.032.05962833
AATCGCG14400.031.35410341
TTGCGGG15750.030.22216250
CACATTC151600.028.76643631
GTCACAT153100.028.53031729
TGCGGGG15850.027.8232951
CAGTCAC158650.027.53225127
AGTCACA160850.027.2427228
ATTCAGA169000.025.90823234
ATGGGGG40450.025.78486842
ATCGGGG17700.025.70616542
ATCGCGT8050.025.65212442
TTCAGAA170400.025.46943335
AATGGGG46950.025.04787441
CCAGTCA176000.024.85790626
ACATTCA177850.024.73708732
CCGGCGT3400.024.70583352
TCAGAAA174050.024.63367736
Produced by FastQC (version 0.11.5)