FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Pool_L2_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Pool_L2_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTGGTGTTTCGCCGAGGGAGGGTTCCCCGGTGGTTCGGCGCGGGGGG14.3478260869565215No Hit
AAATAATTTTTCAATTATTCATAGAAATAAGCACTAAACCGCGCTAAATA14.3478260869565215No Hit
CTAAAGATAAAAAAACTGAAACAATTTATGTGTACTATATAAAACATTCA14.3478260869565215No Hit
TTATCAGTGGAAAGGATGGATTCTTTAAACCTGTGACTCTAGGAGTAGCT14.3478260869565215No Hit
AAATAATCAGGCACAATTATAGTTTGGGGATTGAATTGTCTAAGCAAACG14.3478260869565215No Hit
GTTATTGAGTCACTTTCAGATTGCTCTTAATTTTCTGTGTGGGGTTGTGG14.3478260869565215No Hit
AACCAAGGTTATTGCTAAAGGGAATTACTGAATAACTTAGTAAAATAGAG14.3478260869565215No Hit
ATCTTATAAGCCTAAGTAGTTTTTACTTGTTAGAATAAAAAAATCAAAAT14.3478260869565215No Hit
GTAGATGATACCTCAGTAATGGTCGGCATAGCTTAATTGCGGGGGACTTA14.3478260869565215No Hit
GATTCCTGAGATTATGCAAGGCAGCATAAATCTACCTGTGGATGATAGAA14.3478260869565215No Hit
GAGAGACCATCAAGCAATACGGCTCTGGACGAGATATTGCCTTGTACAGA14.3478260869565215No Hit
GTAATATGATAATAGAGAGAGACTCATTTTAGGAAATGGCATAGCAAATG14.3478260869565215No Hit
TTCACTACAAAAAAAATAATATAGTAATTTTCCAAAAAGGTTTTTGGTTC14.3478260869565215No Hit
CTTTATATGCTCGAATGGTTTGTTGGAATATCCCACATAACATCTAGCTA14.3478260869565215No Hit
TAAGGAAAATAATTGATTTTGAGGACATTGGAGATCTAAGCAGAAAGGGA14.3478260869565215No Hit
GTTTGCTTAGTTAACATACCATTTACAGAACAAGAATGTAACAATCGATT14.3478260869565215No Hit
ATTTATTATATATATGCAGCTCGATGACATCGCCGTTTGTTCAGTGATGT14.3478260869565215No Hit
TAATAAAATGAAGTATCCAGGCTCCGTTTAGCAAAAACCCAATTGATAAT14.3478260869565215No Hit
CGAGCGGAGACTATATATTTTTTTTTAAAAAAAATCCTTATAAAATATAA14.3478260869565215No Hit
CATACACATGAGATCAAGTCATATTCGACTCCAAAACACTAGATCGGAAG14.3478260869565215No Hit
CCCAACAGCTCAAGATCCTGAAATTAAGCAACTTATTCAATAAATCATTC14.3478260869565215No Hit
TTTTTTCACCAAAGAAATCATTTTAATATAGATGCAGTTTTTTATATTTA14.3478260869565215No Hit
ATCCTTCATCCTTTTTGATTTGAAGTTTCGATCGAAGGATTCCTTTATGA14.3478260869565215No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)