FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Pool_L1_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Pool_L1_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134
Sequences flagged as poor quality0
Sequence length76
%GC40

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATTGTTTCCACGTCAGCAGGGTCGTCTCCTGCGAATGAGTTAGATC10.7462686567164178No Hit
CAAGAAGAAGAAAAAGAAGCCACTTCATGAACCCATCAACTCCATGTAGT10.7462686567164178No Hit
TTCCACACGATTGATTCGCCACGCAGATGTTGCAATAAGCGGTCGCGTTT10.7462686567164178No Hit
GGAACTACCTGCCACGACCACAACATACATTTGCTCACCCAACCGTCAAT10.7462686567164178No Hit
CATAAGGAGGCAAAGAGCGAGATACTCTTCTTCAGAAGGAGGAGGGTTTG10.7462686567164178No Hit
GAGGGCTGGGCTCGGGGGTCCCAGTTCCGAACCCGTCGGCTGTCAGCGGA10.7462686567164178No Hit
GGCGACCAAATTCAGTGACGAACGTGGCGGCCAAACCGGCCGCGTTGACA10.7462686567164178No Hit
CTCAACACCTGAAAACCACATTTTATCGAAAAGCTCAAACGCTTCACTCC10.7462686567164178No Hit
ATATGAAATACCGAAGGTTAATTTGAGTAATTGTACTTTTTAAACAGAAA10.7462686567164178No Hit
TACACAAAGATCATGCAAGGCTATGCATCAGTCGGTGATACCGGAAAAGC10.7462686567164178No Hit
GACAAATCAAGCCGAAGCACGTATCGAACCACACGAAGAAGTGTCTGCAG10.7462686567164178No Hit
CGCTAACGCCACTATGTATTTTTTGACAGCTACATTTATGTTTCTTTAAC10.7462686567164178No Hit
TTTGTATCTTCTAACAAGAAAACACTACTTAGGCTTATAAGATCGAGTTG10.7462686567164178No Hit
GGTTGTTTCCCAGTTTATTATTTTGTTGATTACCACAGAACTGGTCCTGT10.7462686567164178No Hit
GGCTTTGTGTCTTCTAACAAGGATACACTTCTTAGGCTTACAAGATTGGG10.7462686567164178No Hit
AGTGGCTGTACTTCCTTGGACCCACAACTGATATCACATCTTTTAGAATG10.7462686567164178No Hit
GTCTTCATCAACCAAGTTTTGAGTATCCAATGCCATAGTTCTGATACAAA10.7462686567164178No Hit
AAACTAATCAGTTCAATATCCTTTTAAGCTGAGTTAGTCTCCCGACCCAA10.7462686567164178No Hit
CTCAACAAAATAAAAAGCAAACTGATTAACCCCCTAAAACCCAACTCAGA10.7462686567164178No Hit
TAATAGCTGAAGATAACTTGAGATAAGCTGAAGGTAAGAGATACACAATT10.7462686567164178No Hit
TGAAACGCGAGAGTAGATCGTCGTGTATGTGGTGTGGTACACTATGTTTA10.7462686567164178No Hit
GTCCATATGAGTCTTTGGCTTTGTGTCTTCTAACAAGGATACAATTCTTA10.7462686567164178No Hit
GTAGGGCAGGGAAGATTAATGAAGCATATGAAATACTGAAGCGGATGGAT10.7462686567164178No Hit
ATCTCACACGCATTTCTACCGGGAACTGCAGCAAGTCTCTCAAAGCTATC10.7462686567164178No Hit
TTTATTCCAAAAACAAACTCCTCTACCAAGTTACTACCACCATGACTTCT10.7462686567164178No Hit
AGAGCTAGCTGGTTCTCCCCGAAATGCGTTGAGGCGCAGCAGTTGAGTGG10.7462686567164178No Hit
CCATAAGAATTAGGACACGTGGGCACTGGATATTGGGTACTCATCAGTCC10.7462686567164178No Hit
GTCTAAACGATGCCGTTTTGGGGTTTGGAGAAGAAGAAGACAAACGCGAC10.7462686567164178No Hit
AGTCAGTAATCGATGCAAATTTGATTCCAACCTTGGTCAAACTGACTCAG10.7462686567164178No Hit
TAGGAAGACGCGTGAATCTGATTTACACGCGTCTTCCACAATTTCTTCAG10.7462686567164178No Hit
GGATGTGCAATATCGTGACATCGTTGTTTAGGCGAAGCATTCGTGCGTTC10.7462686567164178No Hit
ATACTAGATCTGACCAACTGACCATACTACCTACTCTACGTTCTTGACAG10.7462686567164178No Hit
TTAAAGTTTACCCAATCACAACCAAAACTCATCATGATGGTGATGATGCT10.7462686567164178No Hit
ACCACGCTTAGATGTATCTAGCTAGCTACTATTCTAGTGTGTGTATATAA10.7462686567164178No Hit
GGTATTTCGTGATCAAGCCGACTATCCCTCCACCCAAACGGCCTAGGAGC10.7462686567164178No Hit
TATATATATAGAACGTGAATTGTTAACTTTGTTATAAATGGTATAGTTAT10.7462686567164178No Hit
GCCCATAGGACAAAAATAATCAGCAGGTGGAAGGGAGATTGTATTCTTGT10.7462686567164178No Hit
TAATTCGGTGATATGACTTCGAATAAAACAGCAATACAATGTTTATATCT10.7462686567164178No Hit
ATTGCTGATATAATAACCTCTATACGAAATGCTGACATGAATAGAAAAGG10.7462686567164178No Hit
GACTTGTCATCGCACAGATCATGTCATGGCTGAGTTATTCTAACATAACT10.7462686567164178No Hit
GTTAATCTCCGTTGGAGGTAATAAAAATAGAAAGAAATAGAGAGTGAGGT10.7462686567164178No Hit
TAAGTTCCTATGAAGAAGCACTTATGATATTAGAAATTATGCCTTATCGA10.7462686567164178No Hit
AGACTCCACACAAATTATATTTTACTCGTAATACTTTTATTGTTGACGTT10.7462686567164178No Hit
GCTCTGCCCATATGTCATCCATCAGGTAAACAGCTTGCAAACTTCCACCT10.7462686567164178No Hit
GCCACATGCATCGTTGTTAACAACGAATGACGTCGGCTTAACGCATTAGC10.7462686567164178No Hit
AGTGACATAGAAGAGGATGATGGTGCGTGGAGTGCAAAGGCTGGTTACGG10.7462686567164178No Hit
GAAACCGGGCACCCACGGTATGCCGCGTCGTGCTATGGGCGCCGTCACAG10.7462686567164178No Hit
GGCATTGCCCGTCTCCCAAAAGGACCCCATTTACAAATGGCGTTGTCGAG10.7462686567164178No Hit
GGGTTTAGGTTTTGGGGTGTGGGATTTTGGGGTTAGGGGTTAGGCTTAGT10.7462686567164178No Hit
TACTCTTCCTACAACAACTTCACCATCATGGGCTCTTCATCCTCCGACAC10.7462686567164178No Hit
TATACTCAATCAGACACGTGACATCAAGTCATATTCGACTCCAAAACACT10.7462686567164178No Hit
ATGCTGATGTAGAGAAAATCTTACTAAGTATGATCCAAGTAAGGCTAGGT10.7462686567164178No Hit
GTTTCCGTGTCAAACAATATTATCCACTAACACTTCAAACAACTTTCGCA10.7462686567164178No Hit
CCTATCGAATACCATATAAAATAGAACTATGTTAGAAAAGGATAGAATTA10.7462686567164178No Hit
GGAATGAATGTCAGTCTTATGTGGAACTCTGTTGGTTTTTATCAGGTATT10.7462686567164178No Hit
AAACAATAGAGATTATTAACCCAACGACCTCAATACTGCTAAACTGAATG10.7462686567164178No Hit
AGCGAGCAACGTAAACAGACATTATTGATGCTATGAAAACAAAATATAAG10.7462686567164178No Hit
GATTCTTCGTTTTTGACACCAAATTGTCTTAGCATATGTGACCTGTATAG10.7462686567164178No Hit
CTTTGCTAATTTGCTTGTCTCAAGTGGAGTACTCAAGACTTACGAATCCG10.7462686567164178No Hit
ATCGTTCTGACTATTATGATTCTGGGAGATGCATCAACGGTGTTGTATAT10.7462686567164178No Hit
CTTATTGATGCTAAACGAGCTCAGAGGACATGCATGTGAGTCATACGACC10.7462686567164178No Hit
AGGCAAGTAAGGTATACATACCCAGCTGTTATAGCTTCTGAATACCCATT10.7462686567164178No Hit
GGTTCCAACCCACAGCGTTGCGGCTGGACTAGGATCGATAGAAGGAGTAG10.7462686567164178No Hit
GGGAGAATCGTGACGTGTCACTACCAAAGCCGTTCCTTCATTTGGATACC10.7462686567164178No Hit
GGCTTTGTGTCTTCTAACAAGGATACACTACTTAGGCTTTTAAGATCGAG10.7462686567164178No Hit
ATGTATGGATCAGATGTTGAATCACAGATAACTTTGAATCTTCCAACAGA10.7462686567164178No Hit
GACTAGGACCAATAGGTCAAAAATTGGTTTGTGCCGCAGGTGTTGAACGA10.7462686567164178No Hit
GGATGTATAATAAAAAACTATTTAAAAAAAAAAACAAAATATATAACATG10.7462686567164178No Hit
CAAGTTCTGCCATGCGGTTCATCTAATTTGCTCTTGCACCTGAACCAATG10.7462686567164178No Hit
GTTTGCGCTTCGGAGTCAAATATGAATAGATTTCTTGTGTATCATTGACT10.7462686567164178No Hit
ATATATAGAATTAGATTTTTTTAGAAACTGTTTTTTGTTTGAAAAAGTAT10.7462686567164178No Hit
AATATTCTTTATTATATCATTATCGAGACTACAAAAAGTGTTTTATAGGG10.7462686567164178No Hit
GCACGTGTAAACGAGGTGCTCTACCTAACTGAGCTATAGCCCTTGTGTTT10.7462686567164178No Hit
TTTAGCTGTAATGTTTTGATTTCAATAAATAAAATTTAGGCAGGCCACAA10.7462686567164178No Hit
TCGATCACGTACTAACCCGCAACTCTTCTATTCTTAGAACAGTTTAGGTG10.7462686567164178No Hit
CTCTATTGTATGTTTACAGGAAATGCCTATAATCTATTTGCTTTCCCCTC10.7462686567164178No Hit
AATAATATCGACAAGTACAGACATCCCTTCACCAGATGACTTGGATTCTG10.7462686567164178No Hit
CCATTTTCTTGTCGAGTTAATGGATTGGGTGAAAGATGACGATACCAAGA10.7462686567164178No Hit
TGTATCCTTGATAGAATACACAAAGCCAAAGAATCATAAGAACATTGGCT10.7462686567164178No Hit
CCTGCTCGCCCGCGTCGCGCTCGCCGAACGGACACCGGACACGGACGCAG10.7462686567164178No Hit
TGAATGGCCTGTGAGTGCTGACGTATACACTTTTGTCCACGTCAGCATCA10.7462686567164178No Hit
TTGTCTTAATGCACCTGCAAATACACGTCATCATATTATCTCATTTGTGT10.7462686567164178No Hit
GATTAGAGCTTGCAGACAGCATTATAACTAAGTAACAATTATATAATAAC10.7462686567164178No Hit
GGATAGGAGGAGCTTGACCATACACCAGCTCAAATGGTGTCATCTGACTA10.7462686567164178No Hit
TAAGTCTGGTCCGAAAAAATTGTGTGCATAAAGCCCTGGTTACAAAATTG10.7462686567164178No Hit
TTCCCAAAGCGAGGTAAATGCCACGTCACTTCACATAAGCCAAACTCCGA10.7462686567164178No Hit
GAGAGGCAAGACACTAGCTTTTATAGGCGATTCAGTAGCTCATAATCAGA10.7462686567164178No Hit
AACGGGTTTGATGACGTTTCTGTGCCACAATTACAGTTATCTGCGGATTA10.7462686567164178No Hit
TATCCACACAGGGCTCTGCTTGCACTCCACAACAAGACATGTCTACATTA10.7462686567164178No Hit
TACTAATCTAAGAATAGATGACTATACATGTAACACTTTGGAGTCTCGAA10.7462686567164178No Hit
TCCATGTCAGCCCACGCATGACACGCCCATTCTTCTCTGCCACTCAGCAA10.7462686567164178No Hit
TTCTGATCACATCTCATCTGTTTGCATCACACCCATTTGGATTGACACCC10.7462686567164178No Hit
TTCCTGTTATGACGTGGCAACGAAGAGTCAAAATAGCTCTTGGTGCAGCC10.7462686567164178No Hit
TTGTGTGTTAATCTGTAGAACAAGAATCAAGGAGTATCTTCCCATTGGTG10.7462686567164178No Hit
GATAGACATTGCGACAGAGATGGTGATCTCCGAGCTGAAGCGTCCGCCGT10.7462686567164178No Hit
CAAGGGACACTATTTGTTATTTATTCATGAAACTAAAAAACAAAATAAGA10.7462686567164178No Hit
GAAAACACGTCTATTTCTAGAGGTCATGATTATTCTGCTTCCTGGACCAA10.7462686567164178No Hit
GACATATGTAATCTCGATTGAGGTTCGTTCCTGTATATTTCTCAGATCGG10.7462686567164178No Hit
AATATTCGAATCATATACAGAAAAGGATGACATGTAGAATAGAAGTAATT10.7462686567164178No Hit
CTGATCAGATAACGGAATTCAATTAAACCCGTTGAACTGTTCTCTTAGAG10.7462686567164178No Hit
ACTCATATGGACTTTGGCTACACCATGAAAGCTTTGAGCTGTAATAAGAA10.7462686567164178No Hit
GCCGGAGCAATGCCGCGTGGAGGTAGAAGGCCTACGGGTCCTGAACTTCG10.7462686567164178No Hit
TCTATAATTTCGCATGTGAGGGGTTATTTCTTCGTTTCGTCCAGTCATTA10.7462686567164178No Hit
AGACACGTGTCTCATCCACCATGTGCTTACTAAGTTACTATATACCATTT10.7462686567164178No Hit
GGATGTGATATGGTTCCGAAGGATGAACTGGATATGGACAGTTCCAATAA10.7462686567164178No Hit
GAAGAAAAACCAAGAAAAAAAATGATTATTTAGTAGAATATATTTAAGGA10.7462686567164178No Hit
GCTGGAGTATGATCCGTCCATGTGTATTATAACTGAATCATCGACAGTAT10.7462686567164178No Hit
CATTGCTGGTGGCGCGTGTATAACCGTCCAGTACGTGACCAACATCATCA10.7462686567164178No Hit
ACCTTCTTCTTGCTTCTCAAAGCTTTCATGGTGTAGCCAAAGTCCATATG10.7462686567164178No Hit
ATACACATCTTTCATTTTTAAAAGTAAACAATGTTTAATTATTTTTTATT10.7462686567164178No Hit
GCGCGTTTGGCAGTTATCTTCTCGTGCAAGCCTGGTCTCCAGAATTTGAC10.7462686567164178No Hit
AAACTCATAACTCTTGAGAGCTTTTCTCATCGTGAGATAGTTTGCTTACA10.7462686567164178No Hit
GGCATTTAGTACTTTGTTAGTTAAATGATGCAGTCGAGAGAGGAGATCAG10.7462686567164178No Hit
TTTGAGTATTTAAAATACGTACGGATAAAGGGTAAATCCGAAATTTAGAG10.7462686567164178No Hit
CAGACATAGACATTTGGTTTCTCTAATCGGATTTTGCGATGAAAACAAAG10.7462686567164178No Hit
CTAATAAACAACACATGTCTCATCCAAAGACTAAGCATCATAATCATAAA10.7462686567164178No Hit
TACACGTATCACATTTAGGTTACATATTACTCTTTCGAGCTACACAGTAT10.7462686567164178No Hit
ATGAGTCTTTGGCTTTGTGTCTTATAACAAGGATACAATTTTTACGCCTA10.7462686567164178No Hit
AAAGAGACGAGCGGTCGGATCCATGAATGTGTTTGGGTCCTACTTTTTCA10.7462686567164178No Hit
ATGCAGTTGCGGTTTAAGTTCTTATACTCAATCATACACATGAGATCAAG10.7462686567164178No Hit
GTATCATTTCAAAAATTTTGCCTAGACAGGATATGCCTTATTTGCAAGAC10.7462686567164178No Hit
GGCCTTGACACGTGTCAAACTAAATCAGGCCCATTTTATTTTCGCGTGGC10.7462686567164178No Hit
AAAAAAGGGCTTTCCTTTCCTGAGATTTCCGGTGATAAGTCATTGACTTT10.7462686567164178No Hit
AGCTATCTGGCTCGATCTCGTTCGTCCACGTAGACGATTTAGCTCGTGCG10.7462686567164178No Hit
TCCAATGTCCTCCTCCACCCCGTCCTTCAGTCCCAAGTCCTAATCCTAGG10.7462686567164178No Hit
TACAGGTAAATTGATATATGTATATTATAGAATAATTAATTATCATTAAA10.7462686567164178No Hit
TATATCAAACAATATGATAAAACGTCACAAAAGCTATCACTCGCCGTTGA10.7462686567164178No Hit
GGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGTTGGTTAGTGTTT10.7462686567164178No Hit
GGACAGCTCATATATTCATAGCATCTCTATTTGACCATTCCAATGCAATG10.7462686567164178No Hit
TAAATGTTATTGAGCATGGCTGATAAAACCTACACGTCACAGAATCGAAA10.7462686567164178No Hit
GTCCTCTCCAATCGCATCAACGTAAGTCTCCATTTTTTACTCCGTACGTT10.7462686567164178No Hit
AAATTTATAAAAGTTAATAGACTATGACAAGAAGAAACAAGTAAATGACG10.7462686567164178No Hit
GATCGTTTTCCACTGACATGTTTGCCTTCCAATTTTTGTACGGAGTACCT10.7462686567164178No Hit
ACACATCATCTTCATCATCATCTGATTCTTCAAATTGTTTAACCAATGAA10.7462686567164178No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)