FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1242523
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCT1400.00375419114.9999759
TCGCATA2050.003346722611.9511995
GGCTAGA2150.004776907611.39532966
CTAGACC2300.00788940910.65215529
TGGGAGG2350.00925324610.42551335
GTCTACT2900.0039493119.65515647
CAGTCAC7453.0535375E-88.45636270
GAACTCC15100.07.417206370
CCAGTCA8251.3881145E-67.212108669
CACACGT28600.06.853134670
CAACCCG6753.0744652E-46.74072949
TGAACTC18500.06.621609769
GCACACG30050.06.40597969
TCCAGTC9309.046969E-66.397838668
TCTGAAC19200.06.38019769
CTGAACT17803.6379788E-126.292123370
ACTCCAG11155.684087E-76.27801670
ACGTCTG21750.06.275851770
CGTCTGA20150.06.079394370
CTCCAGT11053.242374E-66.018089367
Produced by FastQC (version 0.11.5)