FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1140114
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCGCT500.00721052327.99985967
CCAGTCA7650.010.98033770
TCCAGTC9300.09.40855569
CTCCAGT10606.184564E-117.924488568
ATCCCTA4000.00609201247.874960419
ACTCCAG10756.85759E-107.48833567
GAACTCC13351.3496901E-96.55427469
CAGTCAC6954.3880762E-46.5467367
GTCTGAA18250.06.328735468
CTGAACT15701.9463187E-106.24200770
TCTGAAC17507.2759576E-115.999970469
TGAACTC15505.9280865E-95.870938368
ACGTCTG19759.094947E-125.848071670
CGTCTGA18051.6370905E-105.817145367
CACGTCT22157.2759576E-125.53044670
CACATTA7650.0090653165.490168669
CACACGT25900.05.270243670
ACAGATC8650.0059977855.260089466
AACTCCA16004.1612475E-75.249973366
AGCACAC30250.05.206585470
Produced by FastQC (version 0.11.5)