FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP63_HALO_bZIP9_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP63_HALO_bZIP9_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4295060
Sequences flagged as poor quality0
Sequence length76
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC10302.2973836E-97.47571170
CCCGCGT5200.00100932647.403829665
CGGGCAG4750.0034209137.36840456
GTATCGG8251.3927238E-67.212104340
GCTACCG5450.00166103997.06420339
TCACGAA38750.07.04514570
CGTATCG9452.1959568E-77.037020739
TGGGGGG7107.627012E-56.9018741
AGCCCGC5100.00682919576.862729563
CGGACAG5300.00989973456.603758335
CTCCAGT62200.06.471046470
AGTCACG46100.06.453347770
GTCACGA41700.06.37888269
AGGGGGG6050.00496978276.3640661
CAGTCAC53600.06.26864270
ACTCCAG66700.06.24436469
GAGCCCG6200.0064164296.20966362
GAACTCC79350.06.175158570
GCCCGCG6450.0096603775.968978464
GGCCATA7150.0042965345.87452271
Produced by FastQC (version 0.11.5)