FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP63_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP63_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1204426
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCGC900.005277043719.44442449
GCAGGAT1600.0090964713.1249859
AGAGCCC2500.001150022411.19998670
CTCCAGT10101.2321834E-87.27721970
CAGTCAC7956.1695628E-67.04401769
ACTCCAG11151.1224984E-86.905821369
CCAGTCA8257.779189E-56.36362968
GTCACAT7752.3848463E-46.322572770
ACGTCTG20050.06.284281370
CACACGT28150.06.092355370
GAACTCC13255.2530595E-86.075464267
AGTCACA9802.030257E-56.071421670
GCACACG28100.05.85408669
CGTCTGA18605.820766E-115.833326370
TCCAGTC9153.2086603E-45.73769867
TCGGAAG50100.05.65867670
CACGTCT22351.8189894E-125.63757769
GTCTGAA19157.585186E-105.48302270
AGCACAC31150.05.393252468
AACTCCA16701.8252103E-75.23951568
Produced by FastQC (version 0.11.5)