FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP53_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP53_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1836727
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCGTC1150.001008335618.26085750
TATGCCG1704.3882043E-516.47057548
ATGCCGT1900.001893143512.89472749
GTATGCC1950.002301192112.56409447
CGTATGC2254.7512617E-412.44443446
CGGAAGC4056.78815E-59.506165570
CAGTCAC16450.08.29786669
AGTCACA18550.07.924522470
CGATCTC5905.8534853E-57.711858340
CCAGTCA17900.07.62569368
GCAAGCT4150.0084746947.590355449
TCCAGTC19600.07.14285270
CTCGTAT5150.0075000436.79611144
GATCTCG5250.0090299166.666661341
ACTCCAG24700.06.659913570
GTCACAT16057.2759576E-126.542051370
TGAACTC35550.06.30097970
TTACTCG7802.59456E-46.28204735
TCTGAAC40150.06.276458369
CTCCAGT22350.06.26397766
Produced by FastQC (version 0.11.5)