FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP25_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP25_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences878585
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACTGC950.00720931818.4210384
CCAGTCA6402.3371285E-78.74999270
TCCAGTC6553.3286233E-78.54961269
AGTCACA6805.884631E-78.23528870
CAGTCAC5109.092337E-58.23528869
CACGTCT16000.07.87499470
CTCCAGT7201.3969639E-67.77777168
ACACGTC17200.07.52906469
ACTCCAG8059.398682E-77.391298367
TCTGAAC12251.9463187E-107.142851469
CACACGT18350.07.05721568
CTGAACT11351.5956175E-86.784135370
GCACACG19400.06.67525267
GTCTGAA14003.8780854E-96.249994868
CGTCTGA13255.2372343E-86.075466667
TCGGAAG36650.05.825370370
ACGTCTG13851.287699E-75.812269770
AGCACAC21700.05.80644766
GAGCACA23800.05.588230670
AGAGCAC25750.05.57281169
Produced by FastQC (version 0.11.5)