FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP25_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP25_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1135048
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGACC1400.00375389814.9999925
CCAGTCA7909.094947E-129.7468370
TCCAGTC8454.0017767E-119.1124269
CTCCAGT9404.0017767E-118.56382568
CAGTCAC6603.3991673E-67.954540370
ACTCCAG9859.240466E-107.817253667
ACACGTC22700.06.938321670
GTCACAT6853.6778612E-46.642331670
CGTCTGA17050.06.568910669
CACACGT24600.06.54471169
CTAGTTT6500.00147450236.461534553
GAACTCC13055.697075E-96.436777665
AGTCACA7752.3843661E-46.32257769
GCACACG25500.06.313721768
AGCACAC28650.05.986034467
GTCTGAA18605.820766E-115.833329770
ACGTCTG18656.366463E-115.81769168
AGAGCAC34750.05.74100370
GAGCACA31200.05.721150466
CTAGATC8050.00256771975.652170755
Produced by FastQC (version 0.11.5)