FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP10_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP10_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences725400
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCAGG500.007208677527.99997320
TTCGCAG657.9565344E-426.9230514
GGTAGCA800.002666222421.87497912
GACTGTC900.00527505619.44442612
ATTCGAG1800.001258497713.61109726
GAGCACC2100.00400467711.66665670
CTCCAGT7901.8189894E-1210.18986370
CAGTCAC5201.1782286E-69.42306867
ACTCCAG8555.456968E-129.41519569
CCAGTCA6059.761225E-78.67767766
TCCAGTC6059.1065685E-68.09916565
GTCACAT5956.481994E-57.647051369
CACATTA5200.00100638857.403838669
GAACTCC10503.3469405E-97.33332667
AGTCACA6955.740892E-57.050352668
TGAACTC11452.564775E-97.03056166
AACTCCA11653.6798156E-96.909864469
ACATTAC5100.00681295156.862738669
TCTGAAC14352.0008883E-116.829262370
GTCTGAA14955.638867E-116.55517869
Produced by FastQC (version 0.11.5)