FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_HALO_bZIP10_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_HALO_bZIP10_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1495156
Sequences flagged as poor quality0
Sequence length76
%GC34

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG1553.5106495E-822.5805848
GTCTGGC952.782002E-422.10520221
ATGCCGT1653.396878E-516.96964849
CGTATGC2454.271953E-614.28567346
ATCAGCG1600.00909791813.12496216
GTATGCC2254.7504282E-412.44440847
TCCCCCC2100.004008133411.66663432
TCGTATG3853.8943304E-59.9999745
CGATCTC4108.0167816E-48.5365640
CTCGTAT3700.00301896228.51348944
ACTCCAG16500.08.06085370
CAGTCAC11102.0008883E-117.88312470
GATCTCG4450.00180250237.86514641
TCTCGTA4050.0068120267.77775643
CCAGTCA12051.6370905E-117.552098369
TACTCGA7056.943033E-56.95056769
GTCACAT11351.6016202E-86.784348569
AGTCACA12703.1814125E-96.614375668
TTACTCG6400.00123773616.562700768
GAACTCC20950.06.515712368
Produced by FastQC (version 0.11.5)