FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_16Y_64x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_16Y_64x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATCCAGGCTTGGTCTATGACTTGAGCATCAATGATTACTTGGACTTCC11.8181818181818181No Hit
GTAATAATTAAACTTGGTGGTAGAATTGTGTTGTTGTTTATTACAATCTT11.8181818181818181No Hit
GGCTGCTAATAAATTCTTTTCTAATTCAACTTCCAATGTTTCAATTTTAT11.8181818181818181No Hit
GTTTGCCCTCGAGTGCGACTGTCCCGCCATCATTCTCACACTTGACTGTT11.8181818181818181No Hit
AATATATGATCTAATCATATACAATTCAGTTGCTTATTATCACAAGTAAA11.8181818181818181No Hit
GTGAGGTTTTCTCAAAAGCTGCTTTGGTTATTAGCCTAAGCGCTGATGTT11.8181818181818181No Hit
TGATCGTAGATAGATATAATTTGCAAACTTTTATATATATTAATTAAAAA11.8181818181818181No Hit
CGATAAGAACGACTTCCTCGCCCGTCTCGACCCCTCGTCTCATCGCCGTC11.8181818181818181No Hit
GTGAGTGATAAGACGAGGCGTCGGCGGATCGGGGGGGTCGGAGGGCGCGT11.8181818181818181No Hit
TATTTAAAATATTGATGCAATAACAAGAAGCATATTATTTATTAAAGCAA11.8181818181818181No Hit
ATTAGTATAATTAATCTATTAATAGCAAGTCCACTTAAAGCCACTGCATT11.8181818181818181No Hit
AAAGCTTAGTTCTTATATATCGTGTAATGTTAAATGCTTGAATTGTATGA11.8181818181818181No Hit
AGGTTTCTCGTAACCCTGAGACGGGTGTAAGCCGAGGATGTGGTTACATC11.8181818181818181No Hit
GAATCGGCAGCGGGTGCTGACTCTGAGTGTGGACGATTTACAGTCCAGGG11.8181818181818181No Hit
ATCACCCCCTTATTGGTGATTTTAAAAACAAAAAAGGTACAATTGGGAAA11.8181818181818181No Hit
GTAGCGAGTGCTATTGGAGTCCCCGTTCATGCAGATCATACGACATTGTT11.8181818181818181No Hit
CACATGTCTGATTATGTTGCGCAGCTTAAAGATGATATATCTTCTCTATA11.8181818181818181No Hit
GATTTAATTAATCCAATCAAGGGTTTGATTGGAGAATTGATAAAGTTTTG11.8181818181818181No Hit
AGCCTCTTGTTCATATCTCTCATCAAAAGGTGCTATTCGATAATGTCTAT11.8181818181818181No Hit
AAAAGTAGTGGAAATAGATTACGATCCCAATCTATGATTAAACTATGACG11.8181818181818181No Hit
TAAACAAAATAGTTACCAATTGCCTAATGTACACCGGGCTGCACCAAACC11.8181818181818181No Hit
GCGCGAAACCCTGCCATCAGAATTGCGCTTCCCCCTAAAAATCCATTTAC11.8181818181818181No Hit
ACCGCATCCATTGACGACAAACTCCGTTCATTATTCGCCTGAGGTAAGCG11.8181818181818181No Hit
GGGGATCTACATTGAGCAACCCATGGGTTATGAAGTGAAGTGATATGAAT11.8181818181818181No Hit
AAACAAAATGTTATTGGCCTCAAAAACATAGAAATAAATAACTATAGAAA11.8181818181818181No Hit
GGAGATTAGGAATAGATGGACTTTCTCTAGGACCTATTTTACTGACCGGA11.8181818181818181No Hit
GATTGTGTTTAGAAGAATATTGTTCGGAAAGTTTGTAAAATCAATAAGAA11.8181818181818181No Hit
AATGCTTGAAAATGACATGAGGTGACCAAACAATTTTGAACTCATACACT11.8181818181818181No Hit
GTGAGGTGTCAGTCAAATCATCTGCATGTACATAAACTGCTAGAATAGAG11.8181818181818181No Hit
GGCATTTTGGCTACCATAAGTCTCTCAGTCGCCTCAAGAAAAGCTTCAAC11.8181818181818181No Hit
ACCGCATAACGAACTATAGATAAGCTGTAAGGTTTATCCTACCCACTTTA11.8181818181818181No Hit
GGCTGTTTCCTCTGTGCTTACCTGCCAAGGGATCTATGACTTTGTACATC11.8181818181818181No Hit
GAACTTTGAACTCTCAAAGTTGTTAAGGATCGGACAACACATATTTAAAT11.8181818181818181No Hit
TGATACAAAAACAAAGCTTGATATGGATAGTCAATTCTTATTCTAGTTCT11.8181818181818181No Hit
AGCGCACCATCAACGTATGATACACAGTAAAACTTTCAATAATTTTAACA11.8181818181818181No Hit
GCTGCTGATAGATCCCGCCCAGTGTCCTCCTCCCTCCCCCGCCGCCAACC11.8181818181818181No Hit
GGATCGTATAAATAAAAGTCTCTAGACCTTAAGGCCCAAAAGAATTGAAA11.8181818181818181No Hit
TTTAGATAAAAATCGAAAATAAACATGATGATGATAAAATCAAGCAAAAG11.8181818181818181No Hit
GAATTTAGAATTCTCGAATTAAGGTTTGAATGTTATACGAGATCTTTTTT11.8181818181818181No Hit
ATAAATTTTAATTTATGCACTCCACCTATATAGCGAAGATTGTGCGTATC11.8181818181818181No Hit
GTTTGTTTAAGTTTGGTTTCTATAAAATTATTCTTATGTCATGCCAAAAT11.8181818181818181No Hit
CAATTCTGCACTTTTCTAACTGCTCCTTCATTATCAATCCTTTTTCTTCT11.8181818181818181No Hit
GGTGCGTTTATTTTGAATTGGGACTGCTGGTTTATTTTGGCTACTTAGCT11.8181818181818181No Hit
GGGCCCGATCCCTGCAACGAAAAATAAATTTTTATAGGGTAGTGTACATA11.8181818181818181No Hit
TTAAGCAATTTATTTATTTTAATTTAAATATTACTAATTAGTTTATTTTA11.8181818181818181No Hit
GGATCGTAACTTAAGAGACACAACAGTAGAAGAAGGACCAAGTACACATG11.8181818181818181No Hit
GTGAGGTATTGAATTAAACATTTTCTTATTTCTATATCTCTTTTTCATTT11.8181818181818181No Hit
ATTATGAAATCCCCTCTCTCCCACTTCACGCCTCGGAACGCACCGTTCTT11.8181818181818181No Hit
GTTAGAGATGACTGAGTTGCCCACTTTTAAGGCTTTTTAAAAGCAATTCT11.8181818181818181No Hit
ACTGTTTTGGCTAAAGGGACATGGTTATAATAAGATATACAATTTATTGG11.8181818181818181No Hit
CGCGCCCGGCGCTGCCTCGAGCTCCCTTGACTATCAGATGTATCTCCGCA11.8181818181818181No Hit
TGTTCGGATTGCTATCATTTTAATGAGATTTTAAAAATTGAAGAAAAAGT11.8181818181818181No Hit
ATGCTCATCTTTATTTTCCAACTGATGGTTCAAGTAATTCCTCATTGTAT11.8181818181818181No Hit
GGGAGGTAAGACCGGAATTATCGCCCGCGGCGAGAAGGCTCTGAATAACA11.8181818181818181No Hit
GGATCGTTTCATAGATTTCTCATTTCCATGCTTTCGCAGAATCATCTTTC11.8181818181818181No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTGA50.070.070
CGCGAAA50.070.02
GAGGCAC50.070.065
AAACCCT50.070.06
AATCCAT50.070.040
AACCGAA50.070.051
ATCCATT50.070.041
GGCACAG50.070.067
TGCGCTT50.070.024
AGAAGTG50.070.057
GAAACCC50.070.05
AAATCCA50.070.039
CCTGCCA50.070.010
CAACCGA50.070.050
CCTAAAA50.070.034
TAAAAAT50.070.036
GAAGTGA50.070.058
AGAGGCA50.070.064
CGCTTCC50.070.026
TTACAAC50.070.047
Produced by FastQC (version 0.11.5)