FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_16Y_4x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_16Y_4x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences645833
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT376650.069.739811
GTGAGGT236600.069.541421
GATCGTA148100.069.196492
GATCGTT97700.068.889452
TGAGGTA108800.068.745412
GATCGTC67550.068.7046662
TGAGGTG40250.068.173912
GATCGTG70550.068.1148152
TGAGGTC49100.067.576382
ATCGTAA59050.067.154953
GAGGTAA43500.067.022993
ATCGTGA25850.067.021273
GAGGTAT38450.066.905073
ATCGTAC18000.066.888893
GGCTCGT11050.066.832581
ATCGTAG27700.066.7148063
ATCGTAT48800.066.342213
ATCGTCT23650.066.0042343
ATCGTCA28000.065.875013
GAGGTAG19600.065.714293
Produced by FastQC (version 0.11.5)