FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_16Y_1x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_16Y_1x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences653974
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT362450.069.783161
GTGAGGT249950.069.599161
GATCGTC66300.069.2608642
GATCGTA145900.069.256262
TGAGGTA111350.068.9626542
GATCGTG64100.068.6348652
GATCGTT92500.068.524252
TGAGGTC51700.068.3074652
ATCGTAA57650.068.2392963
GGCTCGT17750.068.230491
TGAGGTG41950.067.914112
GAGGTAT39700.067.443253
ATCGTAC19750.067.34173
ATCGTAG25150.067.216633
ATCGTAT47250.066.888823
TGAGGTT51700.066.276522
ATCGTGA23700.066.012593
ATCGTGG13650.065.897363
ATCGTCA25550.065.8903353
GAGGTAA43400.065.887033
Produced by FastQC (version 0.11.5)