FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_14Y_64x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_14Y_64x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences719589
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT402950.069.7441941
GTGAGGT260950.069.7096941
GATCGTA159850.069.16792
TGAGGTA117700.069.048362
GATCGTG75800.069.030272
GATCGTC70350.068.756152
GATCGTT103300.068.74632
TGAGGTC53100.068.352092
TGAGGTG43200.068.217522
GGCTCGT25850.067.973711
ATCGTAA64650.067.455463
GAGGTAT42050.067.419673
ATCGTGC9400.067.3935553
ATCGTAG29600.066.8073653
GAGGTAA49400.066.599123
ATCGTGA28650.066.3350143
ATCGTAT50300.066.033733
GAGGTAG22600.065.818523
GAGGTAC12100.065.661093
ATCGTGT25600.065.624933
Produced by FastQC (version 0.11.5)