FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_14Y_4x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_14Y_4x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1080979
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTC17740.1641104961335974TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT463950.069.62281
GTGAGGT290100.069.408831
CCGCATT59250.069.232062
GATCGTA184300.069.107442
ACCGCAT268600.069.074841
CCGCATA114950.069.0256652
CCGCATG44550.068.821552
GATCGTC83200.068.6959152
TGAGGTA139150.068.239312
GATCGTT122350.068.054762
GATCGTG84300.067.924082
TGAGGTC62050.067.405312
ATCGTAA74500.067.369133
CGCATAG21150.066.855793
GGCTCGT13000.066.7692261
TGAGGTG45150.066.666672
CCGCATC71000.066.54932
CGCATAA40950.066.495733
CGCATAT43100.066.183293
GAGGTCT25050.065.9481053
Produced by FastQC (version 0.11.5)