FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_Col_14Y_16x_YQ_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_Col_14Y_16x_YQ_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences863644
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCGCATCGGAAGAGCACACGTCTGAACTCCAGTCACCTGAAGCTATCTC16270.18838780793938242TruSeq Adapter, Index 12 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGATCGT359500.069.717661
GTGAGGT227850.069.493091
CCGCATA91700.069.274812
GATCGTA142100.069.236452
ACCGCAT209500.069.164681
CCGCATT47800.068.682012
GATCGTC62750.068.54982
TGAGGTA105550.068.342012
GATCGTT95800.068.2463462
GATCGTG66200.068.096682
CCGCATG38800.068.0154652
TGAGGTG38600.067.551812
TGAGGTC46750.067.4545442
CGCATAA28600.067.430073
CGCATAG18000.067.277783
CGCATAT36050.066.89323
ATCGTAA59050.066.7993243
GGCTCGT18700.066.631021
CCGCATC52950.066.62892
CCCGCAT9000.066.51
Produced by FastQC (version 0.11.5)