FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL9_Col_B_o3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL9_Col_B_o3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences465479
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACGG604.979836E-429.16662236
CTTGGTG1800.001257317413.6110950
AGTCACG2902.4196714E-512.06894770
GTCACGA3004.4276184E-410.49998470
CAGTCAC3904.4693108E-59.87177970
TCCAGTC4653.0705778E-59.03224568
CCAGTCA4451.8849666E-48.65167268
CTCCAGT5351.7105624E-58.5046667
ACTCCAG5502.1775201E-47.636351666
AAAGTAA6005.9106137E-47.00074151
CTGAACT9057.9273195E-76.961315670
CGTCTGA10301.3392128E-76.79610669
ACGTCTG11502.0525476E-86.695642570
AGAGCAC20400.06.691166470
GTCTGAA11006.242044E-86.681807570
GAACTCC6954.3692297E-46.546752570
AACTCCA7802.578786E-46.28204170
CACGTCT12501.0648182E-76.15999169
AAGAGCA23650.06.067643669
TCTGAAC9852.1796674E-56.040669
Produced by FastQC (version 0.11.5)