FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL9_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL9_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1163351
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAGTCA10500.010.33332170
CTCCAGT11800.09.78812468
TCCAGTC11150.09.7309369
CACGAGA5856.028258E-78.97434867
AGTCACG7051.19162E-88.93615970
TCACGAG6059.785472E-78.67767566
CAGTCAC8757.566996E-108.39998970
ACTCCAG13850.08.3393467
GTCACGA6502.7347887E-68.07691365
GAACTCC18150.07.713489570
ACGAGAT7007.832945E-67.49999168
TGAACTC20300.06.7241369
CTGAACT21100.06.46918668
CGAGATT7055.220017E-46.453892769
CGTCTGA24550.06.130338770
ACGTCTG26250.05.999992469
TCTGAAC22800.05.98683567
AACTCCA18253.45608E-115.94519866
GTCTGAA24850.05.915485470
CACGTCT28500.05.894729670
Produced by FastQC (version 0.11.5)