FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL9_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL9_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2291048
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCGGG1250.001763367316.79992562
TGCCGTC2150.00477909711.39529950
CACGAGA13900.08.0578770
CCAGTCA20450.07.530858570
GTCACGA13655.456968E-127.17976969
TCACGAG14301.8189894E-127.098180369
AGTCACG15400.07.045730669
CGTCTGA49700.06.97210569
CTCCAGT26650.06.829536470
TCCAGTC22350.06.73404569
ACTCCAG29650.06.72876370
CGAGATT14804.5474735E-116.621882469
CAGTCAC18650.06.568622669
GTCTGAA52350.06.485450370
ACGTCTG53950.06.422865468
CTGAACT42050.06.409288469
TGAACTC42250.06.378948770
AAGAGCC9054.166887E-56.188088470
GAACTCC34950.06.10896770
CACGTCT58700.05.90312867
Produced by FastQC (version 0.11.5)