FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL16_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL16_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2395247
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC3001.8189894E-1218.6666647
TATGCCG2952.3646862E-1117.79660448
TGCCGTC2259.411815E-817.11110550
CGTATGC3451.4551915E-1116.2318846
ATGCCGT3658.8457455E-913.42465449
CACACGG2601.17740026E-412.1153870
TCGTATG5502.2717359E-810.18181545
CTCGTAT6352.0849257E-89.37007544
TCTCGTA6959.3605195E-99.06474543
GAGCACC5151.0646207E-58.83494970
TCCAGTC27900.08.78135970
CCAGTCC3650.00267021478.63013570
TATCTCG6908.0195605E-88.62318641
TCCGCCT3700.00302025588.51351120
CTCCAGT31000.07.903223569
CGCTCAT8553.7791324E-87.777775368
CCGCTCA9604.636604E-97.656247669
CCAGTCA22950.07.47276570
ACTCCAG33000.07.4242468
AGTCACC19500.07.35897270
Produced by FastQC (version 0.11.5)