FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL13_Col_B_o2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL13_Col_B_o2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1431176
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCACGA8000.010.49994770
AGTCACG9450.09.99994969
CCAGTCA14150.08.90454970
CAGTCAC12400.08.467770
TCACGAG8655.4151315E-98.09244468
CACGAGA8858.290954E-97.90956569
TCCAGTC15550.07.87777469
ACGAGAT9452.7941496E-87.4073770
GAACTCC24800.06.63303270
CGTCTGA37050.06.612652370
CTGAACT29350.06.558740670
GTCTGAA36650.06.493828370
ACTCCAG20050.06.45882170
CTCCAGT17950.06.43450868
TCTGAAC32450.06.14788970
ACGTCTG39400.06.04057968
AACTCCA26850.05.86589269
TGAACTC29600.05.67564769
CACGTCT43800.05.513671468
ACACGTC46750.05.315481766
Produced by FastQC (version 0.11.5)