FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL13_Col_B_o1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL13_Col_B_o1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2061332
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCACC3601.4613415E-611.66666270
TATGCCG2350.00925662210.42552948
AGTCACG14550.08.9003470
TCACGAG13100.08.81679170
GTCACGA12750.08.50980169
CAGTCAC17500.07.79999769
GCCGAAG4200.0094318187.499997663
ACTCCAG27800.07.42805570
TCCAGTC22400.07.34374770
CTAATCG4850.00418956627.2164923
CTCCAGT25050.07.12574669
CCAGTCA19750.06.911390370
CTGGTGG5100.0068255076.862742443
CTGAACT42250.06.627216370
GAACTCC35500.06.309856470
TCTGAAC45500.06.307690669
CGAGATT13003.5719495E-86.19230669
TAAGAGG6850.00266275326.1313853
CACGAGA12601.2635246E-76.111109367
AGCACAC82400.06.031551470
Produced by FastQC (version 0.11.5)