FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL11_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL11_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1968678
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATGCC2452.4805558E-715.71427947
TGCCGTC2302.2179029E-615.21738650
TATGCCG2604.865542E-714.80768748
GCCGTCT1951.4151615E-414.3589751
ATGCCGT2901.6673639E-613.27585749
CACACGG2659.616773E-613.20754270
GAGCACC5153.6379788E-1212.91261770
GCGCACA2500.001150579811.19999662
CGTATGC3551.5920712E-510.84506746
CCGTCTG3004.4412422E-410.49999669
TCACGAG26300.09.84790570
AGGGGGG3300.00106596099.5456931
GTCACGA29000.09.05172269
AGCGCAC3550.0020749838.87323770
AGATTCC17600.08.74999770
AGTCACG32100.08.72273769
TCGTATG5251.3566911E-58.66666345
CAGTCAC39000.08.61538170
CCAGTCA45500.08.53845970
TCCAGTC51700.08.39458170
Produced by FastQC (version 0.11.5)