FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_ColSPL10_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_ColSPL10_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4015877
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG4600.021.30430848
ATGCCGT4800.019.68746649
GTATGCC5650.017.9645747
CGTATGC5900.016.6101446
TGCCGTC3755.4569682E-1114.93330850
TGGGGGG5200.014.8084041
GCCGTCT3951.875378E-913.29111651
GTCACGC3307.0813294E-611.66679170
CTCGTAT9500.09.5789344
CACGAGA58050.09.52637270
TCGTATG10100.09.3564245
CGAGATT46800.09.19881570
TCACGAG65050.09.09310270
GTCACGA71700.09.07959670
AGTCACG81900.08.71804270
GAGCACC11400.08.59658370
CAGTCAC98100.08.56278270
TGCCGTG5007.236023E-58.39998550
ACGAGAT57600.08.32474270
CATCTCG13000.08.07690841
Produced by FastQC (version 0.11.5)