FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_C24SPL16_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_C24SPL16_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1906761
Sequences flagged as poor quality0
Sequence length76
%GC36

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGCG1901.1345641E-414.73679770
GAGCACC3400.00140054949.26467970
ACCGCTC7604.765752E-109.21049970
CACCGCT7951.1150405E-98.80500569
CCGCTCA6101.1032607E-68.60653170
GTCACCG11100.08.51348870
CAGTCAC13800.07.862295670
CGCTCAT4905.346673E-47.85711967
TCACCGC10554.638423E-107.630308669
AGTCACC13101.6370905E-117.21371969
ACTCCAG22300.06.74885970
CTCGGAA5200.00823426.73074967
CTGAACT33450.06.696542370
CCAGTCA17550.06.581176870
TCTGAAC35800.06.5502669
ACGTCTG43050.06.422744870
TGAACTC33250.06.42103470
TCCAGTC19700.06.395919370
GTCTGAA40350.06.245334670
CGTCTGA39650.06.17904869
Produced by FastQC (version 0.11.5)