FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_C24SPL14_Col_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_C24SPL14_Col_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1492004
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCACCGC7150.012.72725570
GGGGAGG2200.005670423611.13634932
GTCACCG8450.010.76921769
AGTCACC10150.09.31033168
CACCGCT5653.6409747E-68.67255469
CAGTCAC10500.08.66665567
ACCGCTC4905.70566E-58.57141670
CGCTCAT4159.041904E-48.433722570
CCAGTCA11303.6379788E-128.05308666
ACTCCAG16850.07.89316470
CCGCTCA4650.00277558627.526870769
TCCAGTC12805.275069E-106.83592765
TCTGAAC27650.06.83543370
CTCCAGT15652.7284841E-116.485613370
ACACGTC40100.06.2842870
GAACTCC21500.06.186037568
ACGTCTG32900.06.170203770
CGTCTGA30200.06.14237570
GTCTGAA29700.06.12793769
CACACGT43050.05.93495169
Produced by FastQC (version 0.11.5)