FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_C24SPL11_Col_B_wg_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_C24SPL11_Col_B_wg_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6148028
Sequences flagged as poor quality0
Sequence length76
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGCTCATTATCTCGTAT68220.11096240940997666TruSeq Adapter, Index 2 (97% over 37bp)

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG14400.034.99992848
GCCGTCT7900.032.3417151
ATGCCGT14550.031.99306349
TGCCGTC12150.031.97524650
GTATGCC16700.031.64664347
CGTATGC16450.031.489346
CTCGTAT22550.025.14407344
TCGTATG24100.022.07464645
CACCGCG8050.020.43474270
TATCTCG37350.019.11642841
TCTCGTA30500.018.47537243
ATCTCGT38950.016.8934242
AGGGGGG12300.016.2207981
GCCGTCG7850.016.05092451
CCGTCTT17350.015.734838552
TGGGGGG11250.014.9345181
CCGTCGT9800.014.64282952
CCAGTCC17800.014.55053370
ATGCCGG5500.013.99997249
TATCGCG6300.012.77775341
Produced by FastQC (version 0.11.5)