FastQCFastQC Report
Wed 4 Mar 2020
H3T5LAFX2_n01_AtTGA5_Nis_B_o.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T5LAFX2_n01_AtTGA5_Nis_B_o.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5049726
Sequences flagged as poor quality0
Sequence length76
%GC35

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGGT7650.032.02605452
CGGGGTG8550.029.88295653
CATTCAG58850.029.79600733
ATGGGGG19000.026.3420342
TGCGGGG6650.025.789451
CACATTC69150.025.45907631
AATCGCG6900.025.36224641
CAGTCAC73200.024.33736227
AATGGGG23150.023.58524741
TTCAGAA74950.023.48892635
AGTCACA75700.023.48738528
GTCACAT75250.023.44179329
ATTCAGA77800.022.76343234
ATCGGGG7600.022.10520242
ACATTCA80350.021.9974532
ATCGCGG4500.021.77771642
TCAGAAA80400.021.33078436
TCCAGTC84850.021.2020625
CAGAAAT79600.021.06149937
CCAGTCA85150.020.96294826
Produced by FastQC (version 0.11.5)