FastQCFastQC Report
Wed 26 Jul 2017
H3T3WBGX3_n01_nip_12hr_cpa.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T3WBGX3_n01_nip_12hr_cpa.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37946672
Sequences flagged as poor quality0
Sequence length75
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC1279910.33729176566524727No Hit
GTCGGCTGCTAGGCGCCGGCCGAGGCGAGGCGCCGCGCGGAAAACCGCGGCCCGGGGGGCGGACCCGGCGGGGGA1199580.31612258381973524No Hit
CTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCG910160.2398523907445691No Hit
CCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGC887700.23393355812599323No Hit
CCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCGCGCCGCCGCGGGG864680.2278671499835348No Hit
GTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGG801410.2111937510620167No Hit
GTTCAACGTGACGGGCGAGAGGGCGGCCCCCTTTCCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCAC735310.19377456868944923No Hit
CTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGAC598910.157829387515195No Hit
CCCTCCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGG585400.15426912800152803No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC583660.1538105897666072No Hit
CCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCGCGCCG549590.14483220030468022No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT467010.12307008108642573No Hit
CGGGTATCTGGCTTCCTCGGCCCCGGGATTCGGCGAAAGCTGCGGCCGGAGGGCTGTAACACTCGGGGTGAGGTG446910.11777317388992636No Hit
CCGGCCGCACCCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGAC439990.11594956205909177No Hit
CCTACTCGTCGCGGCGTAGCGTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGG434810.11458448846317802No Hit
CCCGTTTCCCAAGACGAACGGCTCTCCGCACCGGACCCCGGTCCCGACGCCCGGCGGGGGGAGACCCCGCGCCGC429540.11319569737235455No Hit
GTGGCGACGACGCGGGGGACGACGGGGCCCCGCGGGGAAGAGGGGAGGGCGGGCCCGGGCGGAAAGGACGGGGGG427360.1126212069400974No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG421580.11109801671145234No Hit
GTCCGCGGGGCCCGACGCCGCGGGGGCGAAACCCGGCGCGCGGAGGGGAGGCGGGGGACGGGCCCCCGCCACGCC412440.10868937333951183No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCC405180.1067761620834628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGTTCG93350.025.9068726
GCCACGC143050.025.75519868
CGTGCGA96650.025.09820238
CGTTCGA99050.024.4828057
GTCGGCT427450.024.1345351
TCGGCTG462350.023.0044692
GTCGGAT101100.022.8311611
GTTGGAC229550.022.1965769
GCGGGTT100150.021.56315665
GCGGACC496500.021.28170259
GCCCCGT67000.021.268081
GCGATCG114550.021.20448741
TAGGCGC508200.021.1502310
GTGCGAT115900.021.13781239
TGCGATC116100.021.01171140
TCGGATT109950.020.92862
CTAGGCG514250.020.8612129
CCCGCCA180100.020.74453565
GTCCGGA52800.020.5186521
GTTCGAA121200.020.3495678
Produced by FastQC (version 0.11.2)