FastQCFastQC Report
Wed 26 Jul 2017
H3T3WBGX3_n01_nip_12hr_con.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T3WBGX3_n01_nip_12hr_con.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44144802
Sequences flagged as poor quality0
Sequence length75
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC646270.14639775709040442No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT617830.13995532248621254No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG514000.11643499952723765No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC462230.10470768449703319No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC323800.022.6198126
AGTCGCC324600.022.401827
GTTGGAC258050.022.3649869
TCGCCGC346900.021.0310239
AGAGTCG370150.020.141645
GCGAAAC219400.019.53531326
AACCCGG223700.019.33111430
ACCGCTA38400.018.7772872
GCCACGC136500.018.67627368
CGAAACC230850.018.65659527
GCCCGAC240550.017.81019210
GTCGCCG411000.017.7257828
GGTTGGA347600.016.93080968
ACGCCGC252250.016.90175415
CCGCCGG426800.016.71332218
CGGGGTT412650.016.52748165
CCGCTAA48250.016.5167853
GTCCAGA487250.016.492431
TCCGCGG257600.016.4329852
GACGCCG260600.016.4263614
Produced by FastQC (version 0.11.2)