FastQCFastQC Report
Wed 26 Jul 2017
H3T3WBGX3_n01_nil_12hr_con.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH3T3WBGX3_n01_nil_12hr_con.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41611321
Sequences flagged as poor quality0
Sequence length75
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGT835960.20089725101493416No Hit
CCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTCCCGCTCCGGAACCCCCGCGGGGTTGGAC724040.17400072446630568No Hit
GTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCCGTTTCCGACCTGGGCCG676080.1624750149124081No Hit
GCCCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCAGCACGGGAGTTTTGACCTGCTCC559270.134403327402175No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCCGAGTGTCCGGGCCCCCCGCCCCACCGGGGGCCCGCTGGTTCCTC532700.12801804585824134No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCAT466760.11217139681770738No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGTCGC372700.023.9008226
AGTCGCC374150.023.842137
GTTGGAC275300.023.71990469
GCCACGC161050.022.31925468
TCGCCGC406950.021.8944539
AGAGTCG421750.021.30935
ACCGCTA39400.020.6647032
GCGAAAC307850.019.54996526
AACCCGG316100.019.12876530
GTCCGCG320750.019.0507351
CGAAACC319200.018.85533727
TCCGCGG318050.018.765672
GTCCAGA509700.018.7442551
ACGAACG123700.018.6838937
CGAACGG123450.018.6374978
CCGCTAA47500.018.5932623
CCCGCCA196900.018.30840765
GCCCGAC331750.018.16486710
GGTTGGA363500.018.12598668
ACGCCGC349250.017.24444415
Produced by FastQC (version 0.11.2)